BLI Technology for Monoclonal Antibody Assay

The use of monoclonal antibodies is gradually changing the landscape of tumor treatment due to their specificity and efficacy. Cell lines capable of secreting highly effective monoclonal antibodies are usually obtained by transient or stable transfer, and cell expression needs to be monitored in real time during cell line selection. Biofilm interference is a non-labeled analytical detection technique based on optical interference signals for rapid detection of biomolecules. The principle is that the bottom end of the biosensor is covered by a biofilm layer, and when the biofilm layer is in contact with another molecule, the thickness of the biofilm layer increases, and after contact, the optical interference spectral shift changes at the sensor membrane layer, and the sample concentration is quantified by monitoring the shift change. CD BioSciences provide BLI technology for monoclonal antibody assay service to help customers complete biofilm related research.

Specific Monoclonal Antibody Assay Approaches We Offer

Specific Antibody Content Detection Approaches We Offer

CD BioSciences has developed a method for high throughput quantitative detection of monoclonal antibody concentrations using Biological Membrane Interference (BLI) technology. We used OctetRed96 molecular interaction analyzer to detect antibody concentration based on the change of optical relative interference shift after binding of the sample to the protein A sensor, and to examine the methodology of this assay. The methodological investigation of this assay was carried out.
The protein A sensor binds specifically to the monoclonal antibody, and we can help determine whether the concentration of the antibody working standard shows a good regression with the binding rate by calculating the following data.

Correlation coefficient
Recovery rate
Relative Standard Deviation (RSD) of Repeatability
RSD of Intermediate Precision

Provided by Clients

Please inform us of your specific BLI technology for monoclonal antibody assay service details, and we can provide customized solutions for different projects.

Sample Requirements

Buffer: PBS, HEPPS, etc. without organic reagents, without Tris
Antibody sample: 200 ug, concentration >0.5 mg/ml

Service Process

Our Features

CD BioSciences provides a flexible and comprehensive approach to all services related to BLI technology for monoclonal antibody assay service.
Our team works with scientists, QA/QC professionals and project managers from many pharmaceutical and biotech companies and organizations around the world.
CD BioSciences will complete your project on time, efficiently and according to your requirements.

Deliverables

Monoclonal antibody assay results
Remaining samples
Experimental report
Data analysis
Summary of relevant calculation parameters

Why Choose Us?

CD BioSciences provides professional biofilm service to meet the needs of regular customers to determine hits on time and on budget. CD BioSciences relies on the world-class technical expertise, we provide customers with the best quality one-stop biofilm service, including the development of experimental procedures according to different experimental needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.

For research use only, not intended for any clinical use.

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