BLI Technology for Antibody Content Assay
Principle of Biofilm Interference (BLI) for Quantitative Detection of Antibodies
Biofilm interferometry (BLI) uses Protein A sensors to quantify antibodies. The thickness of the membrane layer at the end of the sensor changes due to the capture of antibody molecules by Protein A, which is reflected by the displacement value of the interference spectrum, and the graph of the displacement versus time of the interference spectrum provides the kinetic number of antibody molecules bound by Protein A at the end of the sensor. The concentration of the antibody sample can be calculated based on the rate at which Protein A captures antibody molecules in the same time.
Specific Antibody Content Detection Approaches We Offer
The Protein A biosensor was selected and the sample counterpart Buffer was used as the lysis matrix for the preparation of standards and samples. Different concentrations of standards were prepared with the dissolution matrix, and double wells were set up for each concentration; double wells were set up for unknown samples to be tested; the dissolution matrix was used as blank control and internal reference. All standards and samples to be tested were added to a 96-well black plate at 200 μl/well. The samples were assayed by quantitiative assays in the biomolecular interaction instrument.
HPLC and ELISA methods
In addition to the BLI method, we also offer HPLC and ELISA methods for the detection of antibody samples. The BLI, HPLC and ELISA methods are basically the same for the detection of antibody samples; the BLI technique has a high error (CV<5%), rapid detection, high throughput, no sample pretreatment, and is suitable for the screening of antibody samples as well as antibody stock solutions, intermediates and other samples with complex Buffer composition. It is suitable for the screening of antibody samples and the detection of samples with complex Buffer components such as antibody stock solution and intermediate.
Provided by Clients
Please inform us of your specific BLI technology for antibody content assay service details, and we can provide customized solutions for different projects.
Service Process
Our Features
- CD BioSciences has a group of technical experts specializing in biofilm services. Our extensive research and technical experience can help our customers solve their biofilm technical problems.
- CD BioSciences has a proven, advanced biofilm technology platform that is constantly being improved to ensure that we can provide accurate, sensitive, and rapid analytical results for our customers.
- We offer 24/7 round-the-clock service. Relatively faster service efficiency and more competitive service prices in the industry represent our commitment to provide better service to our customers.
Deliverables
- Related experimental results raw data
- Antibody content detection results
- Remaining samples
- Experimental report
- Data analysis
- Image and result analysis
- Summary of relevant calculation parameters
Why Choose Us?
CD BioSciences is a professional biofilm technology provider supporting a wide range of BLI technology for antibody content assay service. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. We have accumulated years of quintessence in the field of biofilm BLI technology, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.
For research use only, not intended for any clinical use.
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