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Pseudomonas Aeruginosa Biofilm Testing

Pseudomonas Aeruginosa Biofilm Testing

Clinically, the number of intractable infections caused by bacterial biofilms is increasing. Biofilms can enhance the ability of bacteria to resist antibiotic treatment and host immune system attack, making clinical treatment more difficult. The formation of bacterial biofilms is a complex biological process. Most previous studies have used post-fixation staining or scanning electron microscopy to observe the morphology of biofilms, and then infer the formation process. All these methods observed the morphological structure of the biofilm in the dead state after fixation, but failed to observe the morphological structure of the biofilm in vivo. Now, CD BioSciences provides you with a variety of Pseudomonas Aeruginosa biofilm testing to meet your scientific research needs.

Our Services

CD BioSciences provides one-stop Pseudomonas Aeruginosa biofilm testing services. We offer a variety of methods and equipment to choose from to meet your research needs. We used the electroporation transformation method to transform the green fluorescent protein (GFP) prokaryotic expression plasmid into Pseudomonas aeruginosa, and then used laser confocal microscopy for real-time detection.

Pseudomonas Aeruginosa Biofilm Testing Approaches We Offer

  • Bacteria Transformed with PCGEX4T-1-GFP Plasmid
    The PCGEX4T-1-GFP plasmid was transformed into each strain of Pseudomonas aeruginosa, and positive transformants were obtained by screening with ampicillin.
  • Biofilm Culture In Vitro
  • Observation and Thickness Measurement of Biofilms by Confocal Laser Confocal Microscopy
    (1) Biofilm observation: Confocal laser scanning microscope was used, horizontally scanned layer by layer, and the step distance was 14um to scan 10 layers.
    (2) Biofilm thickness testing: Scan the biofilm specimen horizontally layer by layer along the Z-axis of the three-dimensional coordinate system from the inside (the surface of the biofilm and the glass slide) to the outside (the free surface of the biofilm), and the level with the smaller Z-axis distance The biofilm structure can be observed on the horizontal layer, and the biofilm structure cannot be observed on the horizontal layer with a larger Z-axis distance, so the Z-axis distance of the latter horizontal layer is the biofilm thickness.
  • Statistical Analysis
    The thickness of biofilm is represented by X, and analyzed by SPSS software; T test was used to compare the mean.

Provided by Clients

Please inform us of your specific Pseudomonas Aeruginosa biofilm testing details, and we can provide customized solutions for different projects.

Service Process

Service Process

Our Features

  • CD BioSciences has a proven, advanced biofilm technology platform that is constantly being improved to ensure that we can provide accurate, sensitive, and rapid analytical results for our customers.
  • CD BioSciences offers a variety of methods to meet your needs. We specialize in biofilm testing project services with very competitive prices and fast turnaround times.
  • With talented people and years of experience, we can fully meet your project requirements and budget in exploring biofilms.

Why Choose Us?

As a professional biofilm service provider, CD BioSciences provides high-quality biofilm testing and technologies to help customers study biofilms more efficiently. Our excellent technology platform can realize a variety of biofilm technology services and solve all difficulties encountered in research for customers. For more details on how we can support your project with innovation, please feel free to contact us.

For research use only, not intended for any clinical use.
Related Services:

CD BioSciences is a specialized company providing biofilm services with expertise in the intersection of chemistry and biophysics. Our platform offers advanced technologies to meet all your biofilm needs.

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