Transcriptomic studies also rely on microarrays and RT-qPCR. With the advent of next-generation sequencing technologies, the entire transcriptome of biofilms can now be analyzed by RNAseq, which is the sequencing of cDNA libraries generated from RNA libraries. Using this RNAseq approach, the number of RNA transcripts per gene can be quantified in cells grown under different conditions, such as in biofilms and planktonic cells. Analysis of the overall expression profile of bacteria when cultured under biofilm and planktonic conditions can help to investigate questions about the physiology of biofilm bacteria. Now, CD BioSciences provides you with a variety of biofilm transcriptomics services to meet your scientific research needs.

Our Services

CD BioSciences provides one-stop biofilm transcriptomics services. We offer various methods to choose from to meet your research needs.

Biofilm Transcriptomics Services Approaches We Offer

• Microarray Research
  For microarray studies, we can isolate total RNA from cells cultured under different conditions. RNA is reverse transcribed into cDNA using reverse transcriptase and hybridized with oligonucleotide probes arrayed on the surface. Fluorescently labeled cDNA is then quantified using single-color arrays or two-color techniques, where cDNAs from different samples are labeled with different fluorescent dyes. Array data are normalized so that direct comparisons can be made between samples. Data from these biofilm studies, as well as other transcriptomic studies, are held in databases.
• Next Generation Sequencing Technology
  We can obtain quantitative descriptions of bacterial transcriptomes using sequencing (RNAseq). The biggest advantage of RNAseq is that the array platform does not need to be available for every organism of interest, making this method applicable to any species of bacteria, archaea, or eukaryotes. RNAseq is suitable for metatranscriptomic studies because prior knowledge of the target organism's genome sequence is not required. It has been applied to a variety of environmental biofilm samples. Another advantage is that RNAseq provides information that may not be available with microarray methods, such as the presence of small noncoding RNAs expressed from intergenic regions and information on promoter sequences and operon structural sites.

Provided by Clients

Please inform us of your specific biofilm transcriptomics services details, and we can provide customized solutions for different projects.

Service Process

Our Features

Why Choose Us?

CD BioSciences has many years of experience in biofilm transcriptomics service and has been a trusted partner to our clients for a long time. If you are looking for these services, you can contact us at any time and we look forward to a pleasant relationship with you.

• Quantitative Characterization of Biofilms
• Qualitative Characterization of Biofilms
• Biofilm Total Protein Determination
• Quantitative Analysis of Bacteria in Biofilms
• Biofilm Genomics and Metagenomics Services
• Biofilm Proteomics Services
• Biofilm Metabolomics Services
• Biofilm TOC Analysis Services
• Interaction Analysis of Biofilm Community
• Biofilm Interface Analysis
• Feature Analysis of Biofilm Formation
• Biofilm Microelectrode Analysis
• Biofilm Interaction Analysis
• Biofilm Volume and Morphology Analysis
• Biofilm ATP Bioluminescence Analysis
• Biofilm Imaging Services
• Biofilm Optical Coherence Tomography Analysis
• Biofilm Optical Density (OD) Analysis