The Ramachandran plot was developed by G. N. Ramachandran et al. in 1963 as a visualization method to describe whether the dihedral angles ψ and φ of amino acid residues in protein structures are in reasonable regions. At the same time, it can also reflect whether the conformation of the protein is reasonable. CD BioSciences provide biofilm ramachandran plots analysis service to help customers complete biofilm related research.
CD BioSciences provides one-stop biofilm ramachandran plots analysis service of biofilm system. We offer a variety of methods and software to choose from to meet your research needs.
The phi and psi torsion angles of the biofilm backbone are two useful parameters for insight into the structural properties of a protein. A plot of phi versus psi is called a ramachandran plots, in which specific regions reflect the properties of protein secondary structural elements or amino acids, while other regions (outside of these regions) are considered forbidden (unreachable). Variation of phi/psi over time can reflect the transformation of the structure. These angles can be calculated by the rama program, although the results are somewhat crude, because The program outputs all angles to a single file. To study individual residues, they can be selected from the graph with the Linux program grep.
We can do a ramachandran plots for a single amino acid, a single protein chain, or even multiple protein chains.
Points about the disallowed area in the ramachandran plots: The disallowed area can be understood as a high-energy area, and only an infinitely high energy can cause a complete disallowance. So there can be occasional points in these areas that are not infinitely high.
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