Gram-negative Bacterial Outer Membrane Construction Service

The cell envelope of Gram-negative bacteria contains a lipopolysaccharide (LPS)-rich outer membrane that serves as the bacterial cell's first line of defense in adverse physical and chemical environments. LPS macromolecules have negatively charged oligosaccharide domains that act as ion brushes, limiting the permeability of charged electrochemical reagents through the membrane. In addition to LPS, the outer membrane has radially extending O-antigen polysaccharide chains and β-barrel membrane proteins, which make bacterial membranes physiologically unique compared to phospholipid cell membranes. Elucidating the interactions of these macromolecular components and their roles in bacterial outer membrane integrity remains a challenge. CD BioSciences use computer-aided methods to provide gram-negative bacterial outer membrane construction service to help customers complete biofilm related research.

Specific Construction Approaches We Offer

To bridge the gap in our current understanding of Gram-negative bacterial membranes, we developed a coarse-grained outer membrane force field that is computationally affordable to simulate dynamic processes on physiologically relevant timescales.
The force field was benchmarked against available experimental and atomic simulation data to obtain properties such as film thickness, residue density distribution, area per lipid, gel-to-liquid crystal phase transition temperature, and ordering parameters.
We will compare the simulated structural and dynamic properties with the corresponding experimental data, and the force field we developed reproduces the overall physiology of LPS-rich membranes.
The affordability of the model we developed for long-term simulations helps determine the mechanistic aspects of the antibacterial effects of chemical agents and facilitates the design of antimicrobial peptides with enhanced outer membrane permeation properties.

Provided by Customers

Please inform us of your specific gram-negative bacterial outer membrane construction service details, and we can provide customized solutions for different projects.

Service Process

Our Features

CD BioSciences specializes in binding mode analysis services and we are committed to contributing to more customers.
CD BioSciences will complete your project on time and efficiently. We have professional after-sales service: which has been established to provide customers with efficient, fast, practical and feasible solutions.
CD BioSciences has in-depth cooperation with many biology companies and research institutes, and has extensive experience in providing them with high-quality products and services.

Deliverables

Related experimental results raw data
Construction results
Image and result analysis
Interaction and movement details
Summary of relevant calculation parameters

Why Choose Us?

CD BioSciences provides professional gram-negative bacterial outer membrane construction service. We will meet the specific needs of customers on time and on budget. CD BioSciences relies on world-class technical expertise, we provide customers with the highest quality one-stop service, including the development of solutions according to different scientific research needs. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.

Reference

Ma H, Irudayanathan F J, Jiang W, et al. Simulating Gram-Negative Bacterial Outer Membrane: A Coarse Grain Model. Journal of Physical Chemistry B, 2015:14668-82.

For research use only, not intended for any clinical use.

Related Services: