Enterococcus faecalis is one of the most frequently isolated bacteria in teeth with failed root canal therapy, and is considered to be an important factor leading to disease after root canal therapy. The biofilm structure of Enterococcus faecalis may make the bacteria more pathogenic and difficult to remove completely, but the specific mechanism remains to be further studied. Currently, it is crucial to establish a biofilm model of Enterococcus faecalis. Now, CD BioSciences provides you with a variety of in vivo model construction of Enterococcus faecalis biofilm service to meet your scientific research needs.

Our Services

CD BioSciences provides one-stop in vitro model construction of Enterococcus faecalis biofilm service. We offer a variety of methods and equipment to choose from to meet your research needs.

In Vitro Model Construction of Enterococcus faecalis Biofilm Approaches We Offer

• In vitro biofilm construction

CD BioSciences established a root tip biofilm model through in vitro experiments, and observed the morphology and structure of the Enterococcus faecalis biofilm model.

• SEM observation

The tissue samples in each group were fixed in glutaraldehyde solution, then dehydrated in graded ethanol, critical point dried, sprayed with gold on the surface of the samples, and observed under SEM.

• CLSM observation

CLSM observed biofilm layer-by-layer images and overlay images, respectively. Under CLSM, bacteria were stained red by PI, and extracellular matrix was stained green by ConA-FITC.

• Image analysis

Photoshop CS5 software was used to calculate the biofilm coverage in the SEM field image magnified at 3 000 times, and the biofilm coverage area on the surface of the CLSM sample was calculated with Leica Q Win software.

• Statistical analysis

SPSS statistical software was used for analysis. The differences in the surface biofilm coverage of SEM observation samples were analyzed by one-way ANOVA. The comparison of means between groups was performed by t test, and the test level was two-sided α=0.05.

Our Advantages

• Mature in vitro biofilm construction process
• Professional biofilm technology platform supports biofilm construction
• Professional after-sales service
• Simple and fast workflow

Provided by Clients

Please inform us of your specific in vitro model construction of Enterococcus faecalis biofilm service details, and we can provide customized solutions for different projects.

Service Process

Our Features

Deliverable

• Results of Enterococcus faecalis biofilm model construction
• Related experimental results raw data
• Data analysis
• Image and result analysis

Why Choose Us?

CD BioSciences offers in vitro model construction of Enterococcus faecalis biofilm service to researchers who wish to benefit from this technology. With talented biofilm technologists and state-of-the-art facilities, we offer integrated, ready-to-use solutions. We are happy to discuss your project and the potential of applying biofilm construction with you. If you have this need, please feel free to contact us.

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