ATP is widely present in various living organisms and is also contained in living bacteria, which will be decomposed quickly after death by intracellular enzymes. ATP bioluminescence assay is designed based on the luminescence mechanism of bioluminescence system, fireflies have a special luminescent substance - insect luciferin and luciferase, luciferin is easily oxidized. The luciferin is easily oxidized, it is catalyzed by luciferase, activated by ATP, so that it combines with oxygen, and the electrons in the luciferin molecule jump to high energy levels, in an unstable excited state, when the electrons jump back to low energy levels, that is, to emit fluorescent photons. The intensity of this light is proportional to the amount of ATP contained in the substance being tested, and the intensity of the light is measured using a highly sensitive instrument for quantitative analysis.

Overall Solutions

To analyze the bioactivity of bacterial biofilms, CD BioSciences used an ATP bioluminescence assay to determine the ATP content of biofilms. CD BioSciences provides you with a variety of biofilm ATP bioluminescence analysis service to meet your scientific research needs.

Biofilm ATP Bioluminescence Analysis Approaches We Offer

• We provide whether these changes in the studied ATP content have some correlation with the results of scanning electron microscopy assays.
• We can infer the number of viable bacteria by measuring the concentration of ATP in the sample.
• The steps of bioluminescence assay include: cotton swab sampling, sample extraction, addition of luciferin and luciferase mixture, determination of bioluminescence amount, making standard curve, and finding out the concentration and number of live bacteria. Usually, the surface layer of bacteria is wrapped with cell membrane and cell wall, so the sample cannot be measured without treatment. For the determination, the sample should be mixed with the extraction reagent for microorganisms to make the cell membrane and cell wall open, and the ATP extraction reagent is a surfactant-based reagent. Then, the amount of bioluminescence reacting with luciferin and luciferase bioluminescence reagents is measured by fluorimeter.

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Please inform us of your specific biofilm ATP bioluminescence analysis service details, and we can provide customized solutions for different projects.

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Why Choose Us?

CD BioSciences has been insisting on in-depth research on biofilms and has hired a large number of biofilm technology experts to form a professional team. We aim to solve the problems encountered in the process of researching biofilms for our customers, and provide various biofilm technology services including biofilm ATP bioluminescence analysis service. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project. For more details on how we can support your project with innovation, please feel free to contact us.

• Quantitative Characterization of Biofilms
• Qualitative Characterization of Biofilms
• Biofilm Total Protein Determination
• Quantitative Analysis of Bacteria in Biofilms
• Biofilm Genomics and Metagenomics Services
• Biofilm Transcriptomics Services
• Biofilm Proteomics Services
• Biofilm Metabolomics Services
• Biofilm TOC Analysis Services
• Interaction Analysis of Biofilm Community
• Biofilm Interface Analysis
• Feature Analysis of Biofilm Formation
• Biofilm Microelectrode Analysis
• Biofilm Interaction Analysis
• Biofilm Volume and Morphology Analysis
• Biofilm Imaging Services
• Biofilm Optical Coherence Tomography Analysis
• Biofilm Optical Density (OD) Analysis