In Vitro Model Construction of Saprolegnia Biofilm
Biofilm is a three-dimensional community of bacterial cells attached to a biotic or abiotic surface and surrounded by an extracellular polymorphic matrix (ECM) produced by the BF itself. Microorganisms can interchange between planktonic and multicellular communities, with biofilm providing shelter for the bacterium and acting as a source of persistent infection. A variety of pathogenic fungi have been shown to form biofilm similar to bacterial biofilm, and the formation of biofilm has a strong influence on the environmental adaptability and drug sensitivity of the fungus. Saprolegnia is a conditionally pathogenic fungus widely found in freshwater, with a wide range of temperature adaptations and can occur throughout the year. Now, CD BioSciences provides you with a variety of in vitro model construction of Saprolegnia biofilm service to meet your scientific research needs.
Our Services
In 2013, Ali reported for the first time that Saprolegnia can form biofilm and survive in large numbers in biofilm (even after the application of effective drugs), thus posing a persistent infection to aquatic animals[1]. Therefore, it is very important to investigate the pathogenicity of Saprolegnias and to develop drugs for the prevention and control of Saprolegnias. CD BioSciences provides one-stop in vitro model construction of Saprolegnia biofilm service. We offer a variety of methods and equipment to choose from to meet your research needs.
In Vitro Model Construction of Saprolegnia Biofilm Approaches We Offer
We placed sterile coverslips and polyethylene mesh in the culture plate, and each material was placed in 3 wells. The spore suspension and glucose liquid medium were added to the plates. Screen the plates for in vitro construction of BF hydromycetes.
- Quantitative analysis of BF
The suitable materials were selected according to the above method, and the BF was constructed in the plates according to the same method, and a blank control group was set up without the addition of A. hydrophila spore suspension. Determine the OD450nm value. The results were expressed as the mean plus or minus standard deviation, using The results were analyzed statistically using EXCEL and SPSS software.
Our Advantages
- Short turnaround time
- Adaptable to high throughput workflows
- Simple and fast workflow
Provided by Clients
Please inform us of your specific in vitro model construction of Saprolegnia biofilm service details, and we can provide customized solutions for different projects.
Service Process
Our Features
- CD BioSciences has established a proven biofilm technology platform for a variety of applications. We'll help you design the right biofilm technology service to aid your scientific research.
- CD BioSciences is a leading biofilm technology company offering a trusted suite of services and solutions, especially with our extensive experience in biofilm testing.
Deliverable
- Results of Saprolegnia biofilm model construction
- Service quality inspection report
- Other experimental data you need
Why Choose Us?
CD BioSciences is committed to providing customers with comprehensive biofilm construction services to help customers study biofilms more efficiently. Our excellent technology platform can realize a variety of biofilm technology services, and the praise from customers all over the world is the proof of our high-quality services. If you would like to know more about this service, please feel free to contact us.
Reference
- Ali S E, Thoen E, Vralstad T, et al. Development and reproduction of Saprolegnia species in biofilms. Vet Microbiol, 2013, 163: 133-141.
For research use only, not intended for any clinical use.
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