Total protein determination is a widely accepted surrogate for total biofilm growth is total protein content. Assuming that protein content is roughly similar between cells, protein content has been found to correlate with the number of cells in biofilms in wetland miniature biofilms. However, variability in protein yield across species, age, and culture conditions can lead to deviations from direct correlations with cell numbers, which allows this method to be used in conjunction with tight experimental controls and validated by more direct quantitative methods. Now, CD BioSciences provides you with a variety of biofilm analysis service to meet your scientific research needs.

Our Services

Biofilm total protein determination is a rapid, commonly used assay that allows a relative assessment of biofilm growth. CD BioSciences provides one-stop biofilm total protein determination service. We offer the following methods to choose from to meet your research needs:

Biofilm Total Protein Determination Approaches We Offer

• Bradford Method
  The Bradford method consists of adding a known volume of protein sample to acid Bradford reagent containing Coomassie brilliant blue G-250 dye. Add lysed sample or standard protein to Bradford reagent and incubate for a short period of time at room temperature. During this incubation, the protein binds to the dye, causing the spectrum to change from brown to blue. Protein binding depends on the presence of positively charged amino acids in the protein structure that interact with the net negative charge through van der Waals forces, ionic and hydrophobic interactions dye interactions. Therefore, the change in absorbance at 595 nm was measured and converted to total protein concentration by a BSA standard curve.
• Lowry Method
  Lowry protein analysis is based on redox chemistry in two steps.
• First, protein samples were incubated with copper sulfate and tartrate for ten minutes at room temperature to form a tetradentate copper complex consisting of four peptide bonds and one copper atom.
• In the second step, the Folin phenol reagent is added, and when incubated at room temperature for 30 minutes or 30 minutes, electrons are transferred to the phosphomolybdenum/phosphotungstic acid complex in the Folin phenol reagent to make the tetradentate copper complex pale. The blue color is enhanced and the final color absorbs optimally at approximately 750 nm.
• BCA Method
  The chemical mechanism of the BCA protein assay is very similar to the Lowry assay, which utilizes protein reduction of copper ions to cause spectral shifts.

Provided by Clients

Please inform us of your specific biofilm total protein determination service details, and we can provide customized solutions for different projects.

Service Process

Our Features

Why Choose Us?

CD BioSciences is a professional biofilm technology provider, supporting a variety of biofilm analysis services. We are able to perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. CD BioSciences has accumulated years of quintessence in the field of biofilm detection, helping our clients accelerate drug discovery and development and improve the overall success rate of their projects. For more details on how we can support your project with innovation, please feel free to contact us.

• Quantitative Characterization of Biofilms
• Qualitative Characterization of Biofilms
• Quantitative Analysis of Bacteria in Biofilms
• Biofilm Genomics and Metagenomics Services
• Biofilm Transcriptomics Services
• Biofilm Proteomics Services
• Biofilm Metabolomics Services
• Biofilm TOC Analysis Services
• Interaction Analysis of Biofilm Community
• Biofilm Interface Analysis
• Feature Analysis of Biofilm Formation
• Biofilm Microelectrode Analysis
• Biofilm Interaction Analysis
• Biofilm Volume and Morphology Analysis
• Biofilm ATP Bioluminescence Analysis
• Biofilm Imaging Services
• Biofilm Optical Coherence Tomography Analysis
• Biofilm Optical Density (OD) Analysis