Biofilm-Producing Microorganism Testing
Biofilms are aggregates of organized growth of microorganisms. Bacteria irreversibly attach to the surface of inert or active entities, multiply, differentiate, and secrete some polysaccharide matrix, which is a bacterial aggregate membrane formed by wrapping the bacterial community in it. A single biofilm can be formed by one or more different microorganisms. Now, CD BioSciences provides you with a variety of biofilm-producing microorganism testing services to meet your scientific research needs.
Our Services
CD BioSciences has provided biofilm-producing microorganism testing services for many companies and research institutes. We have a comprehensive and professional biofilm testing technology platform that can provide you with comprehensive services for biofilm testing. The complexity and dynamics of biofilms can be observed by biofilm imaging optical techniques including light microscopy, SEM, TEM, and CLSM. These techniques are used to visualize 3D structures and examine the presence of biofilms.
Biofilm-producing Microorganism Testing Approaches We Offer
- Light microscope
Microorganisms that we can directly observe biofilm-producing using light microscopy include but are not limited to:
We observed acrylic sheets adhered to polymethacrylate films, glass coverslips, and polystyrene petri dishes using light microscopy, respectively. Observation with an optical microscope requires a clear, transparent and flat surface to which microorganisms can attach, without creating a 3D vision of biofilms.
- Transmission electron microscope (TEM)
Due to the insufficient stabilization of polysaccharides by conventional fixatives such as aldehydes, glutaraldehyde, paraformaldehyde, and osmium tetroxide, we removed the moisture of the biofilm by fractional dehydration with alcohol after the fixation step thereafter. After the sample is infiltrated with resin, the sample is embedded in gelatin capsules and polymerized. The sections were then post-stained with uranyl acetate and lead citrate. Monitoring of cellular components can be performed directly in TEM by negative staining.
- Scanning electron microscope (SEM)
The procedure for SEM is similar to TEM with the addition of some chemicals, and the post-fixation and dehydration steps for SEM are similar to those for TEM as in the steps of the TEM method. This step is applied after drying in the dehydration step and coating the sample with gold in processing for SEM, rather than resin infiltration, embedding in gelatin capsules, and lead citrate and acetic acid in processing for TEM Uranyl staining. After dehydration with graded alcohol, the samples were dried and coated with gold palladium. After completing all these steps, observe the sample in SEM.
Provided by Clients
Please inform us of your specific biofilm-producing microorganism testing services details, and we can provide customized solutions for different projects.
Service Process
Our Features
- The scientists in our biofilm laboratory are well-trained in biofilm-producing microorganism testing service and can provide you with a variety of services and customized solutions.
- Our goal is to build a complete biofilm detection technology platform to provide customers with all issues in biofilm analysis.
Why Choose Us?
CD BioSciences is committed to providing customers with comprehensive biofilm detection services to help customers study biofilms more efficiently. Our excellent technology platform can realize a variety of biofilm technology services, and the praise from customers all over the world is the proof of our high-quality services. If you would like to know more about this service, please feel free to contact us. We will ensure to provide you with efficient and high-quality experimental outsourcing services.
Reference
- Krmusaolu S. The Methods for Detection of Biofilm and Screening Antibiofilm Activity of Agents. 2019.
For research use only, not intended for any clinical use.
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