BLI Technology for Drug Screening

Biomolecules bound to the sensor surface form a biofilm that causes interference phenomena to the light waves passing through the sensor. The interference phenomenon is detected in the form of a phase shift, allowing the detection of changes in the number of molecules bound to the sensor surface. CD BioSciences provides professional BLI technology for drug screening to help customers complete biofilm related research.

BLI Small Molecule Screening Method Establishment and Optimization

Biotinylation of target proteins
Solidification of target proteins (SSA Sensor)
Equilibrate in buffer solution to establish baseline
Transfer the Sensor after cured protein to compound gradient dilution solution to start binding
Transfer the bound small molecule Sensor to buffer solution and start dissociation
Repeat the binding-dissociation cycle to the highest compound concentration
Equilibrate the regenerated target protein Sensor in buffer solution
Process data, kinetic fit

In addition, we also offer following services according to your scientific needs:

Single concentration compound library screening
Experimental validation of the concentration gradient of seedling compounds
Multi-target affinity selective single-concentration screening
Compound affinity determination for active proteins

Advantages

High throughput screening
Low sample usage, recyclable
Fast method development, low cost, high throughput screening
Wide affinity dynamic space
Provides kinetic binding and dissociation rate constants
Widely applicable to the whole process of small molecule drug development from early screening of lead compounds to SAR optimization of lead compounds to late validation

Sample Requirements

Buffer: PBS, HEPPS, etc. without organic reagents, without Tris
Protein sample (with antibody): 200 ug, concentration >0.5 mg/ml
Peptide sample: 200 ug, concentration>1mg/ml
Compound small molecule: 200 ug, concentration>1 mg/ml

Provided by Clients

Please inform us of your specific BLI technology for drug screening details, and we can provide customized solutions for different projects.

Service Process

Our Features

CD BioSciences will complete your project on time and efficiently. We have professional after-sales service. We provide customers with efficient and feasible solutions.
CD BioSciences works with scientists from many biotechnology companies. We have extensive knowledge and experience to provide quality assurance services.
We have accumulated many years of essence in the field of biofilm BIL techonology, helping customers to speed up project development and improve the overall success rate of the project.

Deliverables

Related experimental results raw data
Drug screening results
Remaining samples
Experimental report
Data analysis
Image and result analysis
Summary of relevant calculation parameters

Why Choose Us?

CD BioSciences is a professional biofilm technology provider, supporting a variety of BLI technology for drug screening service. We can perform individual tests at the most precise level, as well as design and complete a suite of services as a solution to the subject project. CD BioSciences has many years of experience in BLI technology for drug screening serviceand has been a trusted partner to our clients for a long time. Please feel free to contact us for more detailed information, our scientists will tailor the most reasonable plan for your project.

For research use only, not intended for any clinical use.

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