Biofilm is a highly organized group with structure, coordination and function. The biological characteristics of biofilm bacteria are significantly different from planktonic bacteria. They have stronger environmental adaptability and can resist the action of phagocytes, evade host immunity, especially drug resistance. To further explore the genetic material basis and maintenance mechanism of bacterial biofilm formation, physiological and biochemical characteristics and its regulatory mechanism, biofilm bacteria depolymerization and recolonization mechanism, the relationship between biofilm and the body's immunity, biofilm drug resistance mechanism and a series of issues have a great impact on enriching people's control the means of bacterial infection are of great clinical significance. Now, CD BioSciences provides you with a variety of biofilm drug resistance gene testing services to meet your scientific research needs.

Our Services

CD BioSciences provides one-stop service for biofilm drug resistance gene testing. Our solution steps are as follows:

Biofilm Drug Resistance Gene Test Approaches We Offer

• Drug resistance analysis
  The resistance curve was determined using the standard Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standards Institute (CSLI) guidelines.
• Biofilm formation test
  We analysed biofilm formation with isolates grown under aerobic conditions in Luria Bertani (LB) agar (Condalab) for 24 hours at 37°C to obtain single colonies. Test the biofilm formation assay in 96-well microtiter plates using the appropriate medium, all plates including sterile controls (medium without inoculum) and growth controls (control medium with inoculum). To avoid evaporation, all plates were covered with adhesive foil lids.
• Biofilm quantification
  After incubation, cultures were washed with PBS and dried to complete dryness at 65°C. Biofilms were stained with crystal violet (CV) dye solution and incubated for 10 min at room temperature. Measure the optical density (OD) using a microplate reader.
• Biofilm classification
  In this study, the heterogeneity of sample biomass required defining a cut-off value to classify samples into non-adherent, weakly adherent, moderately adherent, and strongly adherent. For this reason, all samples were tested in triplicate and OD averages were calculated using a negative control (medium without inoculum).
• Statistical Analysis
  Chi-square test and Spearman's rank correlation test by SPSS were used to study the association and correlation between biofilm formation and antimicrobial susceptibility categories and the respective sources of microorganisms.

Provided by Clients

Please inform us of your specific biofilm drug resistance gene testing services details, and we can provide customized solutions for different projects.

Service Process

Our Features

Why Choose Us?

CD BioSciences is a specialized biofilm technology company with the ability to specialize in services such as biofilm analysis in an efficient and precise manner. With outstanding staff, state-of-the-art technology and state-of-the-art facilities, CD BioSciences is able to provide you with a comprehensive range of biofilm detection technologies.

• Biofilm Forming Ability Testing
• Biofilms Antimicrobial Susceptibility Testing
• Biofilm-Producing Microorganism Testing
• Biofilm Fluorescent Labeling Services
• Biofilm Peeling Service
• Biofilm Weight Testing
• Biofilm Matrix Testing
• Biofilm Activity Testing
• Biofilm Active Biomass Testing
• Biofilm Extracellular Polymeric Substance Testing
• Biofilm Exopolysaccharides Testing
• Biofilms Extracellular Proteins Testing
• Biofilm-Embedded Bacteria Testing
• Biofilm-Associated Gene Testing
• Biofilm Testing on Medical Devices
• Identification of Bacteria in Biofilm
• Biofilm Formation-Related Genes Testing
• Anti-Biofilm Activity Testing
• In Vivo Biofilm Testing
• Dialysis Water Biofilm Testing
• Endoscopic Biofilm Testing
• Biofilm and Drug Interaction Testing
• Biofilm Antibacterial Efficacy Testing
• Biofilm Inhibition or Destruction Testing
• Klebsiella Pneumoniae Biofilm Testing
• Biofilm Removal Testing
• Minimum Bactericidal Concentration Testing Services
• Minimum Biofilm Eradication Concentration Assay Services
• Minimum Biofilm Inhibitory Concentration Assay Services
• Minimum Inhibitory Concentration Testing Services
• Biofilm Bactericidal Concentration Testing Services
• Biofilm Prevention Concentration Testing Services