The formation of bacterial biofilm includes the interaction between bacteria and the surface of the attached carrier material and between bacteria and bacteria, which determines and affects the structure, function and composition of mature biofilms; meanwhile, the formation of biofilms is a dynamic process, It mainly includes the following four stages: adhesion and colonization stage, development stage, mature stage and bacterial shedding and replanting stage, the above four stages can be affected by external physical, chemical or biological factors, these factors mainly include nutritional conditions, Metal ion concentration, pH, temperature, etc. It is crucial for the analysis of biofilm formation properties. Now, CD BioSciences provides you with a variety of feature analysis of biofilm formation service to meet your scientific research needs.

Our Services

CD BioSciences provides one-stop feature analysis of biofilm formation service. We offer a variety of methods and equipment to choose from to meet your research needs. We use the crystal violet staining method to screen the strains that produce biofilms from the strains provided by customers, and conduct preliminary research on their film-producing process and characteristics. The research results will provide guidance for the correct and efficient management of biofilms.

Feature Analysis of Biofilm Formation Approaches We Offer

Screening of biofilm-producing strains: Isolate and purify strains by plate streaking, and repeat several times until the colony morphology, size, color, and microscopic morphology (Gram staining) are basically the same.

Biofilm strain identification

Analysis of the dynamic change process of biofilm production: Take a 96-well cell culture plate, add sterile LB medium to the two middle rows of micro-wells, and take out a culture plate every 24 hours and place it under a microplate reader to measure the concentration of planktonic bacteria (OD600), and the amount of biofilm (OD595) was determined by crystal violet staining.

In addition, we used confocal laser microscopy (CLSM) to observe the biofilm structure, and also helped to study the effect of pH on the amount of biofilm produced and the effect of different carbon sources on the amount of biofilm produced.

Data statistics: SPSS 19. 0 software was used for processing, one-way ANOVA comparison to judge the differences between groups, LSD method or Tamhane method for pairwise multiple comparisons, pearson correlation analysis to test the linear correlation between two variables.

Provided by Clients

Please inform us of your specific feature analysis of biofilm formation service details, and we can provide customized solutions for different projects. 

Service Process

Our Features

Why Choose Us?

CD BioSciences is a well-known service provider supporting biofilm analysis. CD BioSciences is committed to perfecting all types of biofilm analysis services. We are able to perform individual tests at the most precise level, as well as design and complete a full suite of services as solutions for thematic projects. For more details on how we can support your project with innovation, please do not hesitate to contact us.

• Quantitative Characterization of Biofilms
• Qualitative Characterization of Biofilms
• Biofilm Total Protein Determination
• Quantitative Analysis of Bacteria in Biofilms
• Biofilm Genomics and Metagenomics Services
• Biofilm Transcriptomics Services
• Biofilm Proteomics Services
• Biofilm Metabolomics Services
• Biofilm TOC Analysis Services
• Interaction Analysis of Biofilm Community
• Biofilm Interface Analysis
• Biofilm Microelectrode Analysis
• Biofilm Interaction Analysis
• Biofilm Volume and Morphology Analysis
• Biofilm ATP Bioluminescence Analysis
• Biofilm Imaging Services
• Biofilm Optical Coherence Tomography Analysis
• Biofilm Optical Density (OD) Analysis